pwallium dcas9 vpr Search Results


pwallium dcas9 vpr  (Addgene inc)
93
Addgene inc pwallium dcas9 vpr
Pwallium Dcas9 Vpr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pwallium+dcas9+vpr/pWalium20-10XUAS-3XFLAG-dCas9-VPR+(Plasmid+%2378897)/pmc08744452-239-0-7
Average 93 stars, based on 1 article reviews
pwallium dcas9 vpr - by Bioz Stars, 2026-10
93/100 stars
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pattb actin5c be2 pactin5c cd dcas9m4 udi attb  (Addgene inc)
92
Addgene inc pattb actin5c be2 pactin5c cd dcas9m4 udi attb
Base editing in Drosophila. (A) Schematic of Drosophila BE2. A catalytically inactive <t>dCas9m4</t> is fused through flexible linkers to CD and uracil DNA glycosylase inhibitor domains. (B) Summary of mechanisms inducing point mutations. A gRNA directs the base editor to a 20 nucleotide target. A cytidine residue may be deaminated, resulting in conversion of the cytidine (C) to a uridine (U). During DNA repair or replication, the uridine has base-paring properties of thymine (T). This results in a C-to-T change on the top strand, or a guanosine-to-adenosine (G-to-A) change if the gRNA targeted the bottom strand. (C) Summary of fly crosses used to induce base editing. In this example, males containing one or more gRNAs (inserted on the second chromosome using the pCFD4 construct)20 are crossed to the base editor (inserted on the X chromosome) that uses the <t>Actin5C</t> promoter to express in all cells. Base editing occurs in male F1 progeny. Editing can be examined directly in F1 males, or mutant lines obtained by crosses to balancers to isolate the mutagenized chromosome. BE2, base editor 2; CD, cytidine deaminase; gRNA, guide RNA; dCas9m4, dead Cas9 containing four mutations. Color images are available online.
Pattb Actin5c Be2 Pactin5c Cd Dcas9m4 Udi Attb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pwallium+dcas9+vpr/pActin5c-CD-dCas9m4-UDI-attB+(Plasmid+%23104879)/pmc08744452-205-2-3
Average 92 stars, based on 1 article reviews
pattb actin5c be2 pactin5c cd dcas9m4 udi attb - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier

Image Search Results


Base editing in Drosophila. (A) Schematic of Drosophila BE2. A catalytically inactive dCas9m4 is fused through flexible linkers to CD and uracil DNA glycosylase inhibitor domains. (B) Summary of mechanisms inducing point mutations. A gRNA directs the base editor to a 20 nucleotide target. A cytidine residue may be deaminated, resulting in conversion of the cytidine (C) to a uridine (U). During DNA repair or replication, the uridine has base-paring properties of thymine (T). This results in a C-to-T change on the top strand, or a guanosine-to-adenosine (G-to-A) change if the gRNA targeted the bottom strand. (C) Summary of fly crosses used to induce base editing. In this example, males containing one or more gRNAs (inserted on the second chromosome using the pCFD4 construct)20 are crossed to the base editor (inserted on the X chromosome) that uses the Actin5C promoter to express in all cells. Base editing occurs in male F1 progeny. Editing can be examined directly in F1 males, or mutant lines obtained by crosses to balancers to isolate the mutagenized chromosome. BE2, base editor 2; CD, cytidine deaminase; gRNA, guide RNA; dCas9m4, dead Cas9 containing four mutations. Color images are available online.

Journal: The CRISPR Journal

Article Title: Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila

doi: 10.1089/crispr.2021.0062

Figure Lengend Snippet: Base editing in Drosophila. (A) Schematic of Drosophila BE2. A catalytically inactive dCas9m4 is fused through flexible linkers to CD and uracil DNA glycosylase inhibitor domains. (B) Summary of mechanisms inducing point mutations. A gRNA directs the base editor to a 20 nucleotide target. A cytidine residue may be deaminated, resulting in conversion of the cytidine (C) to a uridine (U). During DNA repair or replication, the uridine has base-paring properties of thymine (T). This results in a C-to-T change on the top strand, or a guanosine-to-adenosine (G-to-A) change if the gRNA targeted the bottom strand. (C) Summary of fly crosses used to induce base editing. In this example, males containing one or more gRNAs (inserted on the second chromosome using the pCFD4 construct)20 are crossed to the base editor (inserted on the X chromosome) that uses the Actin5C promoter to express in all cells. Base editing occurs in male F1 progeny. Editing can be examined directly in F1 males, or mutant lines obtained by crosses to balancers to isolate the mutagenized chromosome. BE2, base editor 2; CD, cytidine deaminase; gRNA, guide RNA; dCas9m4, dead Cas9 containing four mutations. Color images are available online.

Article Snippet: Cloning of pattB-Actin5C-BE2(pActin5c-CD-dCas9m4-UDI-attB), Addgene# 104879 pWallium-dCas9-VPR (containing Homo sapien codon optimized dCas9m4; Addgene# 78897 19 ) was digested with Nde I/ Eco RI, and a 3.3 kB fragment isolated.

Techniques: Residue, Construct, Mutagenesis